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. 2022 Aug 9;61(17):1743–1756. doi: 10.1021/acs.biochem.2c00104

Table 1. Conditions Used for the Different Aggregation Assays in This Study.

aggregation assay buffer conditions temperature
lipid-induced aggregation 20 mM NaPO4, pH 6.5, 100 μM DMPS 30 °C
surface-catalyzed fibril amplification 20 mM NaPO4, pH 4.8, 50 nM seed 37 °C
fibril elongation 20 mM NaPO4, pH 6.5, 2.5–5 μM seed 37 °C