Fig. 3.

THZ1 suppresses the proliferation and induces the apoptosis of GIST cells in vitro. A Dose–response curves of GIST-T1 and GIST-882 cells after treatment with THZ1 for 72 h and 144 h, respectively. Cell viability was assessed with the CCK-8 assay. B Colony formation assays of GIST-T1 and GIST-882 cells treated with THZ1. C Time-response curves of GIST-T1 and GIST-882 cells upon treatment with THZ1 at concentrations as low as 25, 50, and 100 nmol/L. D Flow cytometry analysis in GIST cells treated with THZ1 at the indicated concentrations or vehicle. E Immunoblotting analysis of cleaved caspase 3 and cleaved PARP protein expression after THZ1 treatment for 24 h