TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| S. meliloti | ||
| RCR2011 | SU47, wild-type | 50 |
| 1021 | RCR2011 Str, Strr derivative of RCR2011 | 38 |
| Rm5000 | rif-5 | 16 |
| RmHY210 | Rm5000 hutV::Ω BglII deletion and insertion | This work |
| RmHY220 | Rm5000 hutX::Ω StuI insertion | This work |
| RmHY230 | Rm5000 hutH2::Ω SmaI insertion | This work |
| RmHZ240 | Rm5000 hutX::lacZ XhoI insertion | This work |
| A. tumefaciens | ||
| GMI9023 | C58 cured of pAtC58 and pTiC58 | 50 |
| At125 | GMI9023 pRmeSU47b | 15 |
| At128 | GMI9023 pRmeSU47a | 15 |
| E. coli | ||
| DH5α | F−supE44 ΔlacU169 (φ80dlacZΔM15) hsdR17(rK− mK+) recA1 endA1 gyrA96 thi-1 relA1 | Bethesda Research Laboratories |
| MT607 | pro-82 thi-1 hsdR17 supE44 recA56 | 15 |
| MT616 | MT607(pRK600) | 15 |
| Plasmids | ||
| pLAFR1 | IncP cosmid cloning vector, Tcr | 18 |
| pRK415 | pRK290 derivative with pUC9 polylinker, Tcr | 31 |
| pRK600 | ColE1 replicon with RK2 transfer region, Cmr | 15 |
| pBluescript SK(−) | Derivative of pUC19 with f1(−)oriR, Apr | Stratagene |
| pGEM −3Zf(+) | Cloning vector | Promega |
| pGM2 | IncP Smr Gmr | 25 |
| pSUP202 | ColE1 Mob+ Tcr Apr Cmr | 52 |
| pKOK5 | Apr Kmr pSUP202 derivative; source of lacZ-Kmr cartridge | 33 |
| pHP45-Ω | Apr pBR322 derivative with interposon Ω Smr/Spr | 48 |
| pLS1 | pLAFR1, S. meliloti 26.5-kb cosmid clone with hut locus | This work |
| pGS4 | 4.0-kb EcoRI fragment from pLS1 cloned into pGEM vector | This work |
| pBS2 | 2.0-kb EcoRI fragment from pBS6 cloned into pBSSK− vector | This work |
| pSUPS2 | EcoRI-EcoRI fragment of pBS2 cloned into pSUP202 | This work |