. 2022 Aug 30;87(18):12041–12051. doi: 10.1021/acs.joc.2c01047

Table 2. Inhibitory Activity Data of Peptidomimetics 15 and 16 in Comparison of SAP-6 on the Interaction spike-ACE2 (Determined Using a Solid-Phase ELISA-like Assay) and on 3CLpro (Determined Using a Fluorogenic Peptide Substrate)^a.

compound	% In(spike–ACE2) at 100 μM	% In(3CLPro) at 100 μM
EDLFYQ (SAP-6)	25%	0%
ED(4b)YQ 15	0%	90–91%
ED(4b)YQ 15	0%	IC₅₀ = 15 ± 6 μM
ED(11b)YQ 16	66–76%	45–57%
ED(11b)YQ 16	IC₅₀ = 20 ± 5 μM	IC₅₀ = 127 ± 76 μM

Mean from three different assays, errors were in the range of 5–10% of the reported values; IC₅₀ values were retrieved from dose–response assays as the concentration of compound required for 50% inhibition, as estimated by nonlinear correlation using GraphPad Prism software.

Table 2. Inhibitory Activity Data of Peptidomimetics 15 and 16 in Comparison of SAP-6 on the Interaction spike-ACE2 (Determined Using a Solid-Phase ELISA-like Assay) and on 3CLpro (Determined Using a Fluorogenic Peptide Substrate)a.

Table 2. Inhibitory Activity Data of Peptidomimetics 15 and 16 in Comparison of SAP-6 on the Interaction spike-ACE2 (Determined Using a Solid-Phase ELISA-like Assay) and on 3CLpro (Determined Using a Fluorogenic Peptide Substrate)^a.