Skip to main content
. 2022 Aug 25;9:976604. doi: 10.3389/fmed.2022.976604

FIGURE 2.

FIGURE 2

COL17A1 editing outcome in RNP and ssODN-treated primary JEB keratinocytes. (A) NGS analysis of the COL17A1 on-target locus presented as a heat map showing indel and HDR pattern of the performed editing strategies. n = 2. Mean value (B) sqRT-PCR analysis of COL17A1 mRNA expression in JEB keratinocytes upon RNP/ssODN treatment. Primary wild-type and untreated JEB cells served as controls. n = 3; Mean ± SEM; Student’s two-tailed t-test. (C) Western blot analysis of cell lysates revealed the presence of restored C17 (180 kDa). β-tubulin (50 kDa) served as loading control. (D) Flow cytometric analysis of C17-stained JEB keratinocytes revealed a high level of C17 restoration. n = 3; Mean ± SEM; Student’s two-tailed t-test. P-values (Significances): > 0.05 (not significant), * ≤ 0.05, ** ≤ 0.01, and * ≤ 0.001.