Autofluorescence depended on resin composition and fluorescence filter
set. BioMed, Clear, and BV007a prints were evaluated in comparison to PDMS for
autofluorescence in Cy5, Rhodamine, EGFP, and DAPI fluorescent channels.
Background subtracted mean grey values were analyzed with ImageJ and used to
determine the fluorescent intensity of each material. Saturation values were at
65,000 AU. Bars show mean ± SD, N=3 intensity measurements per print.
Two-way ANOVA, comparisons shown between resin results versus PDMS for each
respective fluorescent channel, ****p<0.0001.