TABLE 1.

Ammonia channel function and GPAT-GARS coupling

GPATa	GARSa	Temp (°C)	Activity (U/mg)			Channel efficiencyb	Coupling efficiencyc
			Glutaminase	Gln-PRA	Gln-GAR
Ec	Ec	37	54.6 ± 1.85 (3)d	52.4 ± 2.67 (3)	51.0 ± 2.27 (3)	0.96	0.97
Aa	Ec	37	4.24 ± 0.20 (8)	1.42 ± 0.15 (8)	0.41 ± 0.05 (3)	0.34	0.29
Aa	Aa	37	4.24 ± 0.20 (8)	1.42 ± 0.15 (8)	0.26 ± 0.04 (12)	0.34	0.18
Aa	Ec	50	7.36 ± 0.60 (9)	3.90 ± 0.39 (6)	3.21 ± 0.2 (3)	0.53	0.82
Aa	Aa	50	7.36 ± 0.60 (9)	3.90 ± 0.39 (6)	2.63 ± 0.30 (8)	0.53	0.76
Aa	Aa	60	11.8 ± 0.80 (3)	7.63 ± 0.24 (3)	5.84 ± 0.14 (3)	0.65	0.76
Aa	Aa	70	30.4 ± 1.40 (6)	22.6 ± 1.00 (3)	15.1 ± 0.4 (3)	0.74	0.67
Aa	Aa	80	40.5 ± 1.30 (10)	35.4 ± 1.50 (3)	22.4 ± 1.1 (3)	0.87	0.63
Aa	Aa	90	53.2 ± 1.00 (5)	44.7 ± 1.20 (4)	27.9 ± 1.1 (5)	0.84	0.62

^aEc, E. coli; Aa, A. aeolicus. 

^bFraction of NH₃ derived from glutamine hydrolysis (glutaminase) utilized for PRA (Gln-PRA). 

^cFraction of PRA (Gln-PRA) converted to GAR (Gln-GAR). 

^dNumber of repeated measurements.