TABLE 2.
Synergistic inhibition of A. aeolicus GPAT by nucleotides
| Nucleotide
|
I0.5b | Synergismc | |
|---|---|---|---|
| Varied | Fixeda | ||
| AMP | 6.12 ± 1.2 | ||
| ADP | 4.10 ± 0.8 | ||
| GMP | 17.9 ± 2.2 | ||
| GDP | 8.50 ± 1.2 | ||
| AMP | GDP (0.85) | 5.80 ± 1.7 | 1.1 |
| AMP | GDP (4.2) | 1.15 ± 0.2 | 5.3 |
| AMP | GMP (8.0) | 4.77 ± 1.4 | 1.3 |
| ADP | GMP (8.0) | 3.22 ± 0.9 | 1.3 |
| GMP | ADP (2.0) | 9.80 ± 2.0 | 1.8 |
| GDP | AMP (0.6) | 3.30 ± 0.7 | 2.6 |
| GDP | AMP (3.0) | 1.39 ± 0.4 | 6.1 |
a
The millimolar concentration of fixed nucleotide is given in parentheses.
b
The millimolar concentration of varied nucleotide required for 10 or 50% inhibition ± standard deviation.
c
Decreased I0.5 due to the second nucleotide.