Table 1.
Summary table of CRISPR/Cas13 classification.
| Type of Cas13 | Orthologues | Structural Composition | Functional Region | Application Scope | Reference |
|---|---|---|---|---|---|
| Cas13a (1250 aa) |
LshCas13a | HEPN Domains (center and C terminus), pre-CrRNA processing, 3′ Non-G PFS preference (except Lwa and LbuCas13a), DR present on the 5′ end |
ssRNA (spacer length 28–30 nt) |
Virus resistance, RNA knockdown, disease diagnostics | [21,24,26,32] |
| LseCas13a | |||||
| LwaCas13a | |||||
| LbuCas13a | |||||
| LbaCas13a | |||||
| Cas13b (1150 aa) |
BzCas13b | HEPN Domains (N and C terminus), pre-CrRNA processing, 5′ PFS preference of D, 3′ PFS NAN/NNA (Except PspCas13b), DR present on 3′ end | ssRNA (spacer length 30 nt) |
Virus resistance, RNA base editing, RNA knockdown | [17,33] |
| PguCas13b | |||||
| PspCas13b | |||||
| Cas13c (1120 aa) |
FpeCas13c | HEPN domains (center and C terminus), pre-CrRNA processing, No PFS preference, DR present on 5′ end | ssRNA (spacer length 28–30 nt) |
RNA knockdown | [19,28] |
| Cas13d (930 aa) |
RfxCas13d | HEPN domains (center and C terminus), pre-CrRNA processing, No PFS preference, DR present on 5′ end | ssRNA (spacer length 23–30 nt) |
Virus resistance, RNA knockdown, alternative splicing modulation | [34,35] |