Table 2.
Summary table of CRISPR/Cas13 applications in plants.
| Application Scope | Cas13 Type | Plant Species | Target | Reference |
|---|---|---|---|---|
| Viral RNA interference | LshCas13a | Nicotiana benthamiana | Turnip Mosaic Virus (TuMV) | [40] |
| Arabidopsis thaliana | [41] | |||
| Solanum tuberosum | Potato Virus Y (PVY) | [42] | ||
| Nicotiana benthamiana, Oryza sativa | Southern Rice Black-Streaked Dwarf Virus (SRBSDV), Rice Stripe Mosaic Virus (RSMV) | [43] | ||
| LshCas13a, LwaCas13a, PspCas13b, BzCas13b, RfxCas13d | Nicotiana benthamiana |
Turnip Mosaic Virus (TuMV), Tobacco Mosaic Virus (TMV), Potato Virus X (PVX) |
[30] | |
| LshCas13a, LwaCas13a, PspCas13b, RfxCas13d | Nicotiana benthamiana, Ipomoea batatas | Turnip Mosaic Virus (TuMV), Cucumber Mosaic Virus (CMV), Sweet Potato Chlorotic Stunt Virus (SPCSV)-RNase3 | [44] | |
| mRNA knockdown | LwaCas13a |
Oryza sativa ssp. Japonica var. Nipponbare (Protoplasts) |
5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), hydroxycinnamoyl transferase (HCT), and phytoene desaturase (PDS) | [25] |
| LbaCas13a, LbuCas13a | Nicotiana benthamiana, Arabidopsis thaliana, Solanum lycopersicum | PDS transcript | [15] | |
| RNA detection | LwaCas13a, PsmCas13b | (Glyphosate resistant) Glycine max | EPSPS from Agrobacterium sp. strain CP4 (CP4 EPSPS) | [32] |