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. 2022 Sep 2;14(17):4318. doi: 10.3390/cancers14174318

Figure 4.

Figure 4

Flow chart of CAR-NK therapy from preparation of NK cells to infusion of patients. NK cells can be either isolated and expanded from primary sources such as PB and UCB or differentiated from ESC or iPSC. CAR molecules are introduced directly into NK cells or at the beginning of differentiation of iPSC. The prepared CAR-NK cells are sorted and further expanded using either cytokine stimulation or an APC cell (K562-mbIL-21). CAR-NK cells can also be cryopreserved for future use.