Table 1.
Comparison of CAR molecules between CAR-T and CAR-NK.
| Domains | CAR-T | CAR-NK | |
|---|---|---|---|
| TAA binding region | VH-VL, VL-VH | VH-VL, VL-VH, VH-only | |
| Hinge domain/spacer | Non IgG-based: | CD8a, CD28 | CD8a, CD28, DAP12, IgG4, IgG2 CH2-CH3, IgG1 CH2-CH3, IgG4 CH2-CH3 |
| IgG-based: | IgG1, IgG4 | ||
| Transmembrane | CD3ζ, CD28, ICOS, CD8a, CD4 | CD3ζ, CD28, CD8a, 4-1BB, DAP12, TCR ab, CD28-CD3ζ, FceRIγ, murine CD3ζ | |
| ITAM | e.g., CD3ζ, CD28, 4-1BB, CD27, ICOS, OX-40, MYD88, CD40, KIR2DSS2 | e.g., CD3ζ, CD28, 4-1BB | |
Note that, domains of CAR molecules come from corresponding regions of the genes listed here. Examples of motifs used as ITAM for CAR molecule are shown.