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. 2022 Aug 29;11(17):2683. doi: 10.3390/cells11172683

Figure 4.

Figure 4

Tspan8 and CD9 interact with TNFα. (A) TNFα co-localizes with CD9 and Tspan8 at different subcellular compartments in transfected HEK293T cells as assessed by immunofluorescence. (B) Specific interaction of TNFα with the indicated Tspans in transfected HEK293T cells was assessed by FRET microscopy. Data are represented as mean ± s.e.m. n = 9 HPF images (A), n = 3 independent experiments (A,B), * p<0.05, ** p < 0.01, Kruskal–Wallis with Dunn’s post-hoc test (B).