TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Reference |
|---|---|---|
| Strains | ||
| S. mutans | ||
| GS-5 | Wild type, serotype c | 41 |
| BEE | ahpC::Emr::nox-1 | 18 |
| DES | dpr::Spcr | This study |
| KD251 | sod::Emr | This study |
| BEE-DES | dpr::SpcrahpC::Emr::nox-1 | This study |
| KD251-DES | dpr::Spcrsod::Emr | This study |
| E. coli | ||
| DH5α | supE44 ΔlacU169 (φ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | 14 |
| TA4315 | ΔahpC ΔahpF | 36 |
| Plasmids | ||
| pUC118 | AprlacPOZ′ | 44 |
| pACYC184 | Tetr Cmr | 6 |
| pSPC1 | Spcr 1.1-kb EcoRI fragment | 23 |
| pS23 | 5.3-kb fragment containing dpr in pUC118 | This study |
| pS23B1 | 2.6-kb BamHI-BamHI fragment containing dpr from pS23 in pUC118 | This study |
| pS23E12 | 1.8-kb EcoRI-EcoRI fragment containing dpr from pS23 in pUC118 | This study |
| pS23E121 | 0.94-kb EcoRI-EcoRI fragment from pS23E12 | This study |
| pS23E122 | 0.86-kb EcoRI-EcoRI fragment from pS23E12 | This study |
| pD13184 | dpr in pACYC184 | This study |
| pS23B1ES | dpr::Spcr from pS23B1 | This study |
| pS23E12ES | dpr::Spcr from pS23E12 | This study |
| pKD251 | sod::Emr | 27 |