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. 2022 Aug 25;12(17):2188. doi: 10.3390/ani12172188

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effects of green tea extract on intracellular ROS production: ROS level of Caco-2 cells treated with 1, 10, or 100 ppm of GTE and challenged with 500 μM of hydrogen peroxide (H₂O₂) for 60 min. ROS production was assessed by CellROX^® deep red reagent assay and expressed as fluorescence intensity calculated relative to the control group. Values in the graph are means (n = 6) ± SEM. Different letters indicate statistical significance with p < 0.05. 500 μM of hydrogen peroxide (H₂O₂) was used as a positive control. Vitamin E (Vit E) and vitamin C (Vit C) were used as a standard of antioxidant potential.