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. 2022 Aug 25;12(17):2188. doi: 10.3390/ani12172188

Table 1.

Primers used for gene expression analysis.

Gene Primer Sequence (F and R)
5′→3′
Product Length (bp) Accession N. Reference
ZO-1 F: CGGGACTGTTGGTATTGGCTAGA 184 NM_001301025.3 [18]
R: GGCCAGGGCCATAGTAAAGTTTG
OCCL F: TCCTATAAATCCACGCCGGTTC 105 NM_001205254.2 [18]
R: CTCAAAGTTACCACCGCTGCTG
CLDN-2 F: ATTGTGACAGCAGTTGGCTT 86 NM_001171092.1 [19]
R: CTATAGATGTCACACTGGGTGATG
CLDN-3 F: ACATCATCACGTCGCAGAACATC 103 NM_001306.4 [18]
R: AGTGCCAGCAGCGAGTCGTA
CLDN-15 F: TCCTATAAATCCACGCCGGTTC 155 NM_001185080.2 [20]
R: CTCAAAGTTACCACCGCTGCTG
JAM-A F: CAGAGGTGATTCATGGCTCTGTG 96 NM_001382727 [18]
R: TTCCAGGCTGGCAATAACTGAC
RPLP0 F: GCAATGTTGCCAGTGTCTG 142 NM_001002.3 [21]
R: GCCTTGACCTTTTCAGCAA
GAPDH F: TGCACCACCAACTGCTTAGC 87 NM_02046 [22]
R: GGCATGGACTGTGGTCATGAG

F = forward; R = reverse; ZO-1 = zonula occludens-1; OCCL = occludin; CLDN-2 = claudin 2; CLDN-3 = claudin 3; CLDN-15 = claudin 15; JAM-A = Junctional adhesion molecule A; RPLP0 = Ribosomal protein lateral stalk subunit P0; GAPDH = Glyceraldehyde 3-phosphate dehydrogenase.