Table 1.
Gene | Primer Sequence (F and R) 5′→3′ |
Product Length (bp) | Accession N. | Reference |
---|---|---|---|---|
ZO-1 | F: CGGGACTGTTGGTATTGGCTAGA | 184 | NM_001301025.3 | [18] |
R: GGCCAGGGCCATAGTAAAGTTTG | ||||
OCCL | F: TCCTATAAATCCACGCCGGTTC | 105 | NM_001205254.2 | [18] |
R: CTCAAAGTTACCACCGCTGCTG | ||||
CLDN-2 | F: ATTGTGACAGCAGTTGGCTT | 86 | NM_001171092.1 | [19] |
R: CTATAGATGTCACACTGGGTGATG | ||||
CLDN-3 | F: ACATCATCACGTCGCAGAACATC | 103 | NM_001306.4 | [18] |
R: AGTGCCAGCAGCGAGTCGTA | ||||
CLDN-15 | F: TCCTATAAATCCACGCCGGTTC | 155 | NM_001185080.2 | [20] |
R: CTCAAAGTTACCACCGCTGCTG | ||||
JAM-A | F: CAGAGGTGATTCATGGCTCTGTG | 96 | NM_001382727 | [18] |
R: TTCCAGGCTGGCAATAACTGAC | ||||
RPLP0 | F: GCAATGTTGCCAGTGTCTG | 142 | NM_001002.3 | [21] |
R: GCCTTGACCTTTTCAGCAA | ||||
GAPDH | F: TGCACCACCAACTGCTTAGC | 87 | NM_02046 | [22] |
R: GGCATGGACTGTGGTCATGAG |
F = forward; R = reverse; ZO-1 = zonula occludens-1; OCCL = occludin; CLDN-2 = claudin 2; CLDN-3 = claudin 3; CLDN-15 = claudin 15; JAM-A = Junctional adhesion molecule A; RPLP0 = Ribosomal protein lateral stalk subunit P0; GAPDH = Glyceraldehyde 3-phosphate dehydrogenase.