TABLE 3.
Effect of changes in carbon source on PUT2 expression
| Relevant genotype | Sp act of β-galactosidase with growth ona:
|
|||
|---|---|---|---|---|
| Glu | Gly-Eth | Raff | Gal | |
| PUT3 GAL4 PUT2-lacZ | 73 | 75 | 55 | 78 |
| put3Δ GAL4 PUT2-lacZ | 27 | 45 | 32 | 100 |
a
Strains MB838-3C and MB838-2A were grown in minimal media containing glucose (Glu, 2%), glycerol plus ethanol (Gly-Eth, 3% each), raffinose (Raff, 2%), or galactose (Gal, 2%) as the sole carbon source and ammonium sulfate (0.2%), adenine sulfate (20 mg/liter), and uracil (20 mg/liter). For growth on raffinose, these strains were transformed with plasmid pHZ1 carrying the SUC2 gene, and uracil was omitted from the medium. Values are the averages of three determinations; variation was ≤19%.