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. 2022 Sep 1;11(17):2729. doi: 10.3390/cells11172729

Figure 3.

Figure 3

OC-lncRNA is an osteoclast-specific lncRNA localizing to the cytoplasm and positively regulates osteoclast differentiation. (A) Expression levels of OC-lncRNA in osteoclast differentiation and maturation at indicated time points. (B) Relative OC-lncRNA expression levels in various tissues of C57BL/6 mouse and four common mouse cell lines. (C) RNA-FISH for OC-lncRNA in non-treated RAW 264.7 cells. (D) RNA-FISH for OC-lncRNA at indicated time points of osteoclast differentiation. (E) TRAP staining of OC-lncRNA knock-out osteoclasts and control. Quantification of TRAP+ osteoclasts. (F) Representative images of bone resorption assay and quantification of resorption pits. (G) mRNA levels of osteoclastic genes levels detected by real-time PCR. (H) Osteoclastic protein levels detected by western blotting. Error bars represent SD. * p < 0.05, *** p < 0.001.