Figure 5.

CXCR3 positively regulates osteoclast differentiation. The interference efficiency of the siRNAs targeting CXCR3 was detected on mRNA levels by real-time PCR (A) and protein levels by western blotting (B). (C) TRAP staining identified the osteoclast formation after CXCR3 knockdown. (D) Numbers of TRAP+ osteoclasts. Expression levels of genes related to bone resorption (CTSK and MMP9) were detected by real-time PCR (E) and western blotting (F). (G) Representative images of bone resorption assay after CXCR3 knockdown. (H) Quantification of resorption pits. Error bars represent SD. * p < 0.05, ** p < 0.01, and *** p < 0.001.