Table 1.
Description of the different primers and probes used in three separate qPCR to detect E. multilocularis (EM), E. granulosus s.s. (Egss) and E. canadensis (EC).
| Assay Name (Gene Targeted) | Primer/ Probe | Oligonucleotide Sequences (5′-3′) | Product Size | Reference |
|---|---|---|---|---|
| EM (rrnL) |
Forward | CTGTGATCTTGGTGTAGTAGTTGAGATTT | (bp) 84 |
[22] |
| Reverse | GGCTTACGCCGGTCTTAACTC | |||
| Probe | FAM -TGGTCTGTTCGACCTTTTTAGCCTCCAT – TAMRA | |||
| Egss (cox1) |
Forward | AGGGGCTGGTGTTGGTTGGA | 80 | [23] |
| Reverse | TGAAACACCAGCCAAATGCAGAGA | |||
| Probe | FAM – TCCGCCGTTGTCCTCGTCGT – BHQ1 | |||
| EC (nad5) |
Forward | TCTTTCTGATAGACGAGGTTAGG |
109 |
[24] |
| Reverse | TCCATAAAGCCAAAAATTGTAC | |||
| Probe | Cy5 – CGGTGGTTTGTAGTGTGAGTTTGGTG – BHQ2 |