Figure 5.

Dependence of cell functionality on cell type and differentiation state. Cells were cultured for 20 h and metabolic functionality was determined using functional assays. (A–F) Healthy hepatocytes (non-HCC-PHH, N = 4) and PHHs from HCC-diagnosed patients (N = 4) were compared with hepatoma cell lines HepG2 and Huh7 (N = 3). Data are shown as means + SD and were normalized to protein content, n = 3, one-way ANOVA and post hoc Tukey’s test, ** p ≤ 0.01, *** p ≤ 0.001, **** p < 0.0001. Abbreviations: PHH, primary human hepatocyte; PHC, primary human hepatoma cell; HCC, hepatocellular carcinoma.