Table 2.
Summary of antibody dilutions and conditions used in the immunofluorescence analysis of mitochondrial dynamics, mitochondrial biogenesis, synaptic mitophagy and autophagy proteins in mitophagy enhancer-treated and -untreated hAbKI mice.
| Marker Primary Antibody—Species and Dilution |
Purchased from Company, City and State |
Secondary Antibody, Dilution | Purchased from Company, City and State |
|---|---|---|---|
| Drp1 Rabbit polyclonal (#NB110-55288) 1:100 | Novus Biological, Littleton, CO | Donkey anti-rabbit HRP 1:200 | Invitrogen |
| Fis1 Rabbit polyclonal (#10956-1-AP) 1:100 | Protein Tech Group, Inc., Chicago, IL | Donkey anti-rabbit HRP 1:200 | GE Healthcare Amersham, Piscataway, NJ |
| Mfn1 Rabbit polyclonal (#ab191853) 1:100 | Abcam, Cambridge, MA | Donkey anti-rabbit HRP 1:200 | GE Healthcare Amersham, Piscataway, NJ |
| Mfn2 Rabbit polyclonal (#Ab205236) 1:100 | Abcam, Cambridge, MA | Donkey anti-rabbit HRP 1:200 | GE Healthcare Amersham, Piscataway, NJ |
| OPA1 Rabbit polyclonal (#NBP2-59770) 1:100 | Novus Biological, Littleton, CO | Donkey anti-rabbit HRP 1:200 | GE Healthcare Amersham, Piscataway, NJ |
| SYN Rabbit monoclonal (#Ab32127) 1:400 | Abcam, Cambridge, MA | Donkey anti-rabbit HRP 1:200 | GE Healthcare Amersham, Piscataway, NJ |
| PGC1a Rabbit polyclonal (#NBP1-04676) 1:100 | Novus Biological, Littleton, CO | Donkey anti-rabbit HRP 1:200 | GE Healthcare Amersham, Piscataway, NJ |
| NRF1 Rabbit polyclonal (#NBP1-77822) 1:100 | Novus Biological, Littleton, CO | Donkey anti-rabbit HRP 1:200 | GE Healthcare Amersham, Piscataway, NJ |
| NRF2 Rabbit polyclonal (#NBP1-32822) 1:100 | Novus Biological, Littleton, CO | Donkey anti-rabbit HRP 1:200 | GE Healthcare Amersham, Piscataway, NJ |
| TFAM Rabbit polyclonal (#NBP2-59770) 1:100 | Novus Biological, Littleton, CO | Donkey anti-rabbit HRP 1:200 | GE Healthcare Amersham, Piscataway, NJ |
| PINK1 Rabbit polyclonal (#BC100-494) 1:100 | Novus Biological, Littleton, CO | Donkey anti-rabbit HRP 1:200 | GE Healthcare Amersham, Piscataway, NJ |
| Parkin Mouse polyclonal (#NBP2-29838) 1:100 | Novus Biological, Littleton, CO | Sheep anti-mouse HRP 1:200 | GE Healthcare Amersham, Piscataway, NJ |
| Iba1/AIF-12 Rabbit monoclonal (#17178S) 1:100 |
Cell Signaling Technology, Inc., MA | Donkey Anti-rabbit HRP 1:200 | GE Healthcare Amersham, Piscataway, NJ |
| Anti-NeuN Rabbit monoclonal (#Ab177487) 1:100 |
Abcam, Cambridge, MA | Donkey Anti-rabbit HRP 1:200 | GE Healthcare Amersham, Piscataway, NJ |