Figure 6.

Adipocyte-specific HDAC9 gene deletion does not improve metabolic health in HFD-fed male mice. (A) Growth curves of HFD-fed male WT and A-KO mice (n = 11–15). (B) Fat mass and lean mass measured by whole body composition nuclear magnetic resonance (n = 10–12). (C) Tissue weight measurements of SQ, VF and liver at time of sacrifice (n = 10–12). Glucose tolerance (D) and insulin tolerance test (E) quantified by area under the curve (n = 4–5). mRNA expression of (F) adipogenic, (G) metabolic, (H) classic beiging and (I) UCP1-independent beiging genes in SQ from HFD-fed male mice (n = 8). Data represent mean ± SEM. * p < 0.05, ns, not significant. SQ, subcutaneous adipose tissue; VF, visceral adipose tissue; PPARɣ, peroxisome proliferator-activated receptor gamma; Pgc1ɑ, PPARɣ coactivator 1-alpha; C/ebpɑ, CCAAT/enhancer-binding protein alpha; Pfkfb3, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3; Ndfus1, NADH-ubiquinone oxidoreductase; Ndufv2, NADH-ubiquinone oxidoreductase flavoprotein 2; Atp5a1, ATP synthase F1 subunit alpha; Prdm16, PR/SET domain 16; Serca2b; sarco/endoplasmic reticulum Ca²⁺ ATPase 2b.