The workflow and overall clustering results. (A) Baseline information of patients included in this study. (B) The workflow of our study. (C) The clustering of all cells identified 12 main cell types in human great saphenous veins. EC: endothelial cells, FB: fibroblasts, SMC: smooth muscle cells, Neutro: neutrophils, Mast: mast cells, Mac: macrophages, Mono: monocytes, LEC: lymphatic endothelial cells, TNK: T cells and natural killer cells, B: B cells. (D) Cell composition for each patient was plotted. (E) Dotplot shows the signature genes for each cell types. Circle sizes stand for the percentage of cells that expressed genes of interest.