Figure 4.

Graphical illustration of PTX-TRAP depots’ conceptual framework. (A) Synthesis scheme of TRAP PTX: PTX was dissolved in DCM and reacted with succinic anhydride in the presence of DMAP at room temperature to yield PTX succinic acid (PTX-SA). Purified PTX-SA was then reacted with EDC and sNHS in DMF at room temperature, yielding PTX-sNHS conjugate. (B) Following injection, PTX-sNHS conjugate reacted with the amines group on ECM, attaching PTX to the tumour tissues. After the linker moiety dissolved, PTX was released intratumorally. Reprinted and modified with permission from Pandit et al. [106]. 2022, Elsevier.