FIG. 3.

Expression of S. coelicolor catC gene in E. coli. E. coli BL21(DE3)pLysS cells carrying catC-overproducing plasmid pJH3 (lanes 2, 4, and 7) or the parental vector pET21c (lanes 1, 3, and 6) were grown and induced with 1 mM IPTG for 3 h. Cell extracts were electrophoresed on sodium dodecyl sulfate–10% polyacrylamide gels followed by Coomassie brilliant blue staining (lanes 1 and 2), or on nondenaturing 7% polyacrylamide gel followed by catalase activity staining (lanes 3 to 5) or peroxidase activity staining (lanes 6 to 8). Lanes 5 and 8 contained cell extracts of S. coelicolor grown in YEME medium for 40 h as a control. The overproduced CatC protein is indicated by an arrow with the predicted molecular mass (lane 2). Activity bands for catalase-peroxidase of E. coli (HPI) and S. coelicolor (CatC) as well as monofunctional catalase of S. coelicolor (CatA) are indicated.