FIG. 5.

Transient expression of CatC on liquid or solid culture. M145 cells were grown on R2YE plates or in YEME liquid medium for the indicated lengths of time. Mycelial cells grown on R2YE plates were harvested when they formed substrate (24 h), aerial (40 h), or sporulated (86 h) mycelium. The amount of cell extracts analyzed was either 5 to 10 μg for plate cultures or 10 to 20 μg for liquid cultures. The amounts of CatA and CatC protein were determined by Western blot analysis. Either catalase or peroxidase activities were detected on 7% native polyacrylamide gels. Positions of monomeric (CatC-M) and dimeric (CatC-D) CatC protein are indicated.