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Repression of CatC expression by multicopy furA gene. S. lividans cells harboring pIJ702 or pIJ702-furA (pJH7032) carrying the furA gene were grown in YEME medium containing thiostrepton (50 μg/ml) for 25 or 40 h. S1 nuclease mapping analysis was carried out with a 851-bp probe uniquely labeled at the 5′ end of primer CS1. The level of CatC protein was determined by Western blot analysis.
