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. 2022 Sep 1;14(17):4288. doi: 10.3390/cancers14174288

Figure 4.

Figure 4

Effect of VE-821, PF-477736 and MK-1775 on the cell cycle control and its relation to target expression and inhibition: (A) Cell cycle profiles of exponentially growing cells were treated with vehicle control (0.5% DMSO), or 3 μM cisplatin (Cis) ±1 μM VE-821 (VE), 50 nM PF-477736 (PF) or 100 nM MK-1775 (MK) for 24 h before DNA content was analysed. At least 2000 events per sample were recorded. Data are the mean and range of values from two independent experiments. (B) Changes in cisplatin-induced S and G2/M accumulation vs. extent of inhibition cisplatin-induced activation of ATR by 1 μM VE-821, of CHK1 by 50 nM PF-477736 or of WEE1 by 100 nM MK-1775. Data are taken from Figure 2C and Figure 4A. (C) Changes in cisplatin-induced S and G2/M accumulation vs baseline ATR or CHK1 expression in the cell lines. Data are taken from Figure 1B and Figure 4A.