Figure 1.

Neural differentiation of Nestin-ZsGreen hiPSC knockin cell line. After 12 days of neural induction as embryoid bodies (EB), cells started to express Nestin-ZsGreen (A–C). When dissociated and replated, they formed a typical neural rosette structure, while continuing to express ZsGreen (D–F). After FACS for ZsGreen, almost all GFP+ cells expressed SOX1, a well-accepted and specific neural stem cell marker (G–I). The purified NPCs do not contain undifferentiated iPSCs as evidenced by lack of expression of SSEA4 (J–L) and OCT4 (M–O), two commonly used pluripotency markers. Before being grafted to animals, expression of ZsGreen (P–R) was significantly downregulated although Nestin continued to be expressed ubiquitously. Scale Bar: 100 mm.