Figure 3.

Survival of grafted hiPSC-derived neural progenitor cells. Human iPSC-derived NPCs were transplanted at 2 weeks after moderate C5 unilateral contusion SCI (IH 150 kdyne) in nude rats. Robust survival of grafted NPCs was observed in all animals that received hiPSC-NPC grafts at 2 months after transplantation (A,B). The grafted NPCs filled the lesion cavity and primarily remained in areas around the injury epicenter at the injury side (A,B). Grafted hiPSC-NPCs differentiated into cells with the morphology of neurons (C), with long processes extending to the spared host spinal cord (D). Scale bars: (A,B), 500 μm; (C,D), 50 μm.