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. 2022 Aug 25;13:906927. doi: 10.3389/fphar.2022.906927

FIGURE 3.

FIGURE 3

NO production after LPS stimulation and after treatment with DE. Cells were pre-stimulated with different concentrations of LPS for 24 h. Cell supernatant NO production was measured using an enzyme marker (A). Cells were pretreated with DE for 2 h and then stimulated with LPS (500 ng/ml) for another 24 h. Cell supernatant NO production was measured using an enzyme marker (B). Compared with the control group, ### p < 0.001; compared with the LPS group,***p < 0.001.