FIGURE 6.

DE reduction of apoptosis in RAW264.7 cells after LPS stimulation (×100). Cells were pretreated with DE (18.75, 37.5, and 75 μg/ml) for 2 h and then stimulated with LPS (500 ng/ml) for another 3 h or 24 h. Cells were observed using a fluorescent microscope (A); fluorescence intensity recorded by flow cytometry (B); analysis of fluorescence intensity using Image J software(C). Analysis of the proportion of fluorescence intensity (D) and the average fluorescence intensity (E). Compared with the control group, ^### p < 0.001; compared with the LPS group, ***p < 0.001, **p < 0.01.