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. 2022 Aug 25;13:906927. doi: 10.3389/fphar.2022.906927

FIGURE 8.

FIGURE 8

DE reduces neutrophil aggregation and ROS production in zebrafish larvae. Zebrafish larvae from 3 dpf were pretreated with DE for 1 h and then stimulated with CuSO4 (10 μM) for a further 40 min. Fluorescence intensity of neutrophils and ROS were recorded by fluorescence microscopy (A) and analyzed using Image J software for neutrophil aggregation (B) and ROS production (C). Compared with the control group, ### p < 0.001; compared with the CuSO4 group, ***p < 0.001, **p < 0.01.