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The identification FLS–EVs and uptake of FLS–EVs by chondrocytes. (A) TEM observation of the EV morphology. (B) NTA measuring of the EV particle distribution and concentration. (C) WB analysis of the EV markers CD9, TSG101, and HSP70. (D) Representative immunofluorescence images of chondrocytes at various time points, showing the uptake of PKH67 (green)-labeled EVs. Hoechst (blue) staining was performed to label the nuclei.
