Skip to main content
. 2022 Sep 5;11(17):2766. doi: 10.3390/cells11172766

Figure 9.

Figure 9

Validation of the miR-150-3p target and the regulatory effect of miR-150-3p on the innate immune signaling and joint homeostasis. (A) A miR-150-3p binding site on Trim14 and validation of miR-150-3p targeting Trim14 through the dual luciferase assay. (B,C) COLII, ACAN, Trim14, NF-κB, and IFN-β protein levels were analyzed using WB. (D) qPCR analysis of the Trim14, NF-κB, IFN-β, COLII, and ACAN mRNA levels in chondrocytes after miR-150-3p interference. (E) ELISA-based quantitation of the pro-inflammatory cytokines IL-1β, IL-6, and TNF-α in the chondrocyte culture medium. The data are expressed as mean ± SEM from three independent experiments. **** p < 0.00001, *** p < 0.0001, ** p < 0.01, * p < 0.05.