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. 2022 Sep 5;11(17):2766. doi: 10.3390/cells11172766

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Regulatory effect of EVs-150 on AC protection in vivo. (A) Observation of the levels and localization of COLII and ACAN in the AC using IF staining. (B) qPCR analysis of the miR-150-3p expression in the serum EVs after EV treatment. (C) qPCR analysis of the COLII and ACAN mRNA levels in the AC after EV treatment. (D) The COLII and ACAN protein levels were analyzed in the AC using WB after EV treatment. The data are expressed as mean ± SEM from three independent experiments. **** p < 0.00001, *** p < 0.0001, ** p < 0.01, * p < 0.05.