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. 2022 Aug 28;11(17):2677. doi: 10.3390/cells11172677

Table 1.

Sub-cell lines differ in their ciliation capacities.

NIH3T3
Sub-Cell Lines
Ciliated Cells/Total Cells Counted (% of Ciliated Cells).
Cells Were Cultivated in Standard Medium with
10% FCS (NM).
Ciliated Cells/Total Cells Counted
(% of Ciliated Cells).
Cells Were Cultivated in Serum
Starved Medium (SSM).
F2 360/443 = 81.3% 399/533 = 75%
F3 244/458 = 53.3% 317/583 = 54%
E2 237/475 = 50%
B2 214/446 = 48% 333/578 = 58%
E9 224/468 = 47.8%
G3 186/468 = 39.7% 245/487 = 50%
E10 171/556 = 31%
C11 127/475 = 26.7% 235/508 = 46%

Note. Sub-cell lines were grown in NM or SSM, and ciliation was quantified by immunological decoration of primary cilia and manual counting using the LSM (63×).