Table 4.
Decrease in the proportion of ciliated cells after eight weeks of permanent cultivation.
| % | F2 | F3 | E2 | B2 | E9 | E10 | C11 | |
|---|---|---|---|---|---|---|---|---|
| Immediately after the establishment of sub-cell lines | Ciliation | 81.3% | 53.3% | 50% | 48% | 48.8% | 31%% | 26.7% |
| G0/G1 phase | 59.2% | 49.3% | 61.6% | 53.8% | 59.6% | 59.7% | 51.8% | |
| After ~8 weeks of cultivation | Ciliation |
23.6%
(90/364/, 140/610) |
8.5%
(34/508/, 47/447) |
|||||
| G0/G1 phase |
56.4%
(55.1%, 53.3%, 60.7%) |
48.5%
(48.2%,48%, 49.4%) |
Note. Cilia were decorated with anti-acetylated α-tubulin and ARL13B, visualized by LSM, and were manually counted. The ratio illustrates the ciliated cells by the total number of counted cells. Cilia were mainly counted by ARL13B and checked by acetylated α-tubulin. In the case of difference, the average was considered. Cells were seeded at 2.5 × 105 cells in a 6-well plate and cultivated for 24 h in NM. For the FACS assays, the cells were cultivated in NM. % ciliation in blue, % G0/G1-phase in green.