Table 5.
Summary of morphological and molecular characteristics of the F2 and C11 sub-cell lines.
| F2 | C11 | |
|---|---|---|
| morphology | cubic-shaped | spindle-shaped |
| % cells with stress-fibers (by Phalloidin-TRITC-staining) | 78% | 33% |
| α-SMA staining (immune-fluorescence) | +(almost all cells) | most cells negative, only few cells+ |
| α-SMA (Western blot) | strong expression | weak expression |
| ciliation immediately after establishment of sub-cell lines | 81% (in NM), | 27% (in NM), |
| 75% (in SSM) | 46% (in SSM) | |
| nuclear size | ~197 µm2 | ~128 µm2 |
| mitotic index (% cells in metaphase) | 0.80% | 3.60% |
| % cells in G0/G1-phase(FACS assay of cycling cells) | 59.20% | 51.80% |
| ciliation in NM (after ~8-weeks of permanent cultivation) | 24% | 8.50% |
| % cells in G0/G1-phase (FACS assay of cycling cells after ~8-weeks of permanent cultivation) | 56% | 48.50% |
| c-myc expression (qRT-PCR; compared to that of F2 cells) | ↑ | |
| cyclin D1 expression (qRT-PCR; compared to that of F2 cells) | ↑ | |
| doubling time in hours after ~8-months of permanent cultivation | 16.34 h | 14.63 h |
Abbreviations: α-SMA = α-smooth muscle actin; NM = standard medium with 10% FCS; SSM = serum-deprived medium containing 0.5% FCS; qRT-PCR = quantitative reverse transcribed polymerase chain reaction; + positive staining; ↑increased expression. Different colours are used for better readability.