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. 2022 Aug 23;14(17):4083. doi: 10.3390/cancers14174083

Table 2.

The primers and thermal conditions of qPCR analysis.

Target Sequence PCR Thermal Conditions
Ang1 FW 5′-CATTCTTCGCTGCCATTCTG-3′ 95 °C 1 min Initial denaturation
Rev 5′-GCACATTGCCCATGTTGAATC-3′ 95 °C 30 s 35 cycles
60 °C 30 s
Ang2 FW 5′-TTAGCACAAAGGATTCGGACAAT-3′ 72 °C 1 min
Rev 5′-TTTTGTGGGTAGTACTGTCCATTCA-3′ 72 °C 1 min Final extension