Table 2.
Target | Sequence | PCR Thermal Conditions | |||
---|---|---|---|---|---|
Ang1 | FW | 5′-CATTCTTCGCTGCCATTCTG-3′ | 95 °C | 1 min | Initial denaturation |
Rev | 5′-GCACATTGCCCATGTTGAATC-3′ | 95 °C | 30 s | 35 cycles | |
60 °C | 30 s | ||||
Ang2 | FW | 5′-TTAGCACAAAGGATTCGGACAAT-3′ | 72 °C | 1 min | |
Rev | 5′-TTTTGTGGGTAGTACTGTCCATTCA-3′ | 72 °C | 1 min | Final extension |