Table 3.
Vegetable crops’ treatment with PGPB-based biostimulants and observed effects.
| PGPB | Plant Species | Treatments | Observed Effects | Refs |
|---|---|---|---|---|
| Azospirillum brasilense Sp245 | Lactuca sativa L. cv. Elisa | Seeds inoculated with 109 CFU per seed or phosphate buffer, and plants grown under salinity (0–40 mM NaCl) | Increase of plant survival of transplantation at 40 mM NaCl and enhancement of fresh and dry leaf weight, leaf area, chlorophyll and ascorbic acid content | [101] |
|
Azospirillum brasilense Cd (DSM-1843) |
Ocimum basilicum L. cv. Genovese and Red Rubin | Plants inoculated twice with bacteria 106 CFU mL−1 in the nutrient solution and/or with additional 20 mM NO3– or 8 mM SO42− | Additional nutrients but not A. brasilense enhanced fresh biomass. Inoculation increased root growth, unsaturated fatty acids, flavonoids, alkaloids and several terpene derivatives, particularly in Red Rubin. | [104] |
| Azospirillum brasilense and Azotobacter chroococcum (commercial mix) | Ocimum basilicum L. | Inoculation of soil or seed soaking and application to soil (2 l ha−1 with 108 CFU mL−1), bacteria + 50% N or 100% N with and without intercropping with maize | Bacteria application increased fresh and dry yield independently of cropping system. 100% N and bacteria + 50% N were both effective in increasing essential oil (methyl chavicol). | [105] |
| Azotobacter sp., Azospirillum sp., Bacillus licheniformis, B. megatheriumstrain Herbaspirillum sp. and Chlorella vulgaris (commercial mix) |
Lactuca sativa var. crispa L. cv. Santoro and var. longifolia Lam. cv. Quintus |
Application of 0.4 l of bacterial and algal mix per plant every 14 days, for a total of four treatments | Bacterial–algal mix increased the weight of both lettuce varieties but increased total carotenoid and antioxidant activity only in the cv. Quintus (romaine lettuce). | [102,103] |
| Azospirillum lipoferum DO12 and Brevibacillus parabrevis B50 | Lycoperson esculentum Mill. cv. Menhir F1 | Rhizosphere inoculation with 25 g m−2 A. lipoferum (2 × 108 CFU g−1), or B. parabrevis (3 × 109 CFU g−1) | Both bacteria increased tomato marketable yield. A. lipoferum enhanced lycopene, Vitamin C and total polyphenols; B. parabrevis increased mainly polyphenols. | [106] |
| Azospirillum sp. and Azotobacter sp. (commercial mix) | Solanum lycopersicum L. var. cerasiforme | Plastic bag inoculation with 1.4 l of solution prepared with 1 mL L−1 of commercial mix (1.3 × 107 CFU mL−1 of Azospirillum and 5.9 × 107 CFU mL−1 of Azotobacter) with different levels of NaCl (0, 50, 100, 150 mM) | Bacterial mix improved plant growth and yield, fruit dry matter content, pH 4.52, and TSS even under salinity. | [107] |
|
Azospirillum sp.
G. intraradices |
Capsicum annuum L. (Chile Morrón, Pimiento) |
Inoculation with Azospirillum sp. 104 and 106 CFU mL−1 in the nutrient solution at transplant and twice every 30 days, and 25 or 50 spores of G. intraradices at transplant with 50%/100% N and P | Higher concentration of spores and bacteria increased Vitamin C, carotenoids, total soluble solids and acidity; moreover, they improved N and P uptake at reduced N rate. | [108] |
| Azospirillum strains (lipoferum, brasilense, irakense and strain 21) | Foeniculum vulgare cv. Isfahan | Seed-priming with Azospirillum solution (4 mL g−1) × 12 h or microelements | Priming increased seed weight uniformly, essential oil yield, in particular α-pinene and limonene, and in strain 21 also β-pinene but not limonene. | [109] |
|
Rhizobium laguerreae strain HUTR05 |
Lactuca sativa L. var. romaine | Seedling inoculation with 150 µL of bacterial suspension with 108 CFU mL−1 | It increased N and P content, phenolic acids (e.g., dicaffeoyl quinic and cichoric acids) and quercetin 3-O-glucoside flavonoid. | [91] |
|
Rhizobium laguerreae strain PEPV40 |
Spinacia oleracea L. | Inoculation of each seedling at the intersection between roots cotyledons with 250 μL of suspension (108 CFU mL−1) | Increase of spinach leaf number, size and weight, as well as chlorophyll and nitrogen contents. | [84] |
|
Rhizobium laguerreae strain PEPV40 and Bacillus halotolerans SCCPVE07 |
Cichorium endivia L. | Plants inoculated with 2 mL of bacterial suspension (108 CFU mL−1) and irrigated with water containing 0 or 100 mM NaCl | Bacteria promoted plant development even under salinity. They increased K, Fe, Mg, N, phenolic acids (cichoric acid and caffeoyl-tartaric acid) and flavonoids (kaempferol 3-O-glucuronide). | [110] |
|
Rhizobium legiminosarum strain TPV08 Rhizobium sp. strain PETP01 |
Solanum lycopersicum L. var. Cherry Capsicum annuum L. var. Verde Italiano |
Seedlings inoculated with 108 CFU of each strain | TPV08 and PETP01 promoted growth of both tomato and pepper, but particularly pepper fresh weight production and tomato quality (higher N, P, K or Mg). | [90] |
|
Rhizobium etli CE-3, R. leguminosarum SCR R. leguminosarum Semia—4088. |
Solanum lycopersicum L. | Seed priming with 4 mL of each inoculum (108 CFU mL−1) kg−1 seeds + inoculation at 30 DAS with 10% of the covering of the root balls in each treatment | Rhizobia (particularly etli CE-3 and Rl SCR) improved tomato yield, probably by a more efficient acquisition of N, P and K. There were no monetary losses despite the different effects. | [111] |