EPS by AFM on unsaturated P. putida mt-2 biofilms according to sample preparation treatment and age. Before AFM imaging, biofilms were 1 day old, unwashed and desiccated (A), 3 days old, unwashed and desiccated (B), 2 days old, washed and desiccated (C), and 2 days old, washed and undesiccated (D).