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Representative runs without optimized conditions. Column: Resource Q 1 mL (6.5 mm i.d. × 30 mm length). Buffer A: 20 mM NaOH. Buffer B: 20 mM NaOH/2.5 M NaCl, flow rate 5 mL/min, gradient 0−100% B in 20 CV, loading 50 μg (50 μl of 1 mg/mL ISIS 2302) (Reprinted from Ref. [62] Open Access article).
