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. 2022 Aug 23;23(17):9528. doi: 10.3390/ijms23179528

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Cell viability. FACS analysis (a) of U87-MG cultured in the 2D standard condition and in 3DB constructs, with or without Ker-AuNPs, after 24 (left panel) and 56 (right panel) h. The quadrant plots show cells identified by physical parameters using side scatter (SSC-A) and forward scatter (FSC-A). Pacific orange marker was used to detect live cells. Percentage of live cells (b) in the different culture conditions after 24 and 56 h. ** p < 0.002, *** p < 0.001 were considered statistically significant. n = 3 for each experimental group.