Table 1.
Amino acid compositions of TPSPH and five fractions.
| Amount (g/100 g) Dry Basis | ||||||
|---|---|---|---|---|---|---|
| <3 kDa | 3–5 kDa | 5–10 kDa | 10–30 kDa | >30 kDa | TPSPH | |
| Asp | 9.46 ± 1.25 ab | 7.23 ± 0.85 b | 9.48 ± 2.29 ab | 10.09 ± 1.76 a | 8.32 ± 0.24 ab | 8.08 ± 0.17 ab |
| Thr | 2.93 ± 0.24 ab | 1.79 ± 0.26 d | 2.30 ± 0.35 cd | 2.71 ± 0.47 ab | 3.26 ± 0.13 a | 2.50 ± 0.21 bc |
| Ser | 3.74 ± 0.37 b | 0.79 ± 0.08 d | 2.98 ± 0.25 c | 3.79 ± 0.55 b | 5.58 ± 0.12 a | 3.58 ± 0.36 b |
| Glu | 22.91 ± 0.89 a | 11.77 ± 0.63 b | 22.99 ± 1.37 a | 23.10 ± 2.75 a | 20.09 ± 1.82 a | 20.88 ± 1.32 a |
| Gly | 3.94 ± 0.33 a | 4.02 ± 0.27 a | 3.28 ± 0.22 c | 3.47 ± 0.34 ab | 4.19 ± 0.22 a | 3.67 ± 0.18 ab |
| Ala | 3.51 ± 0.25 b | 5.86 ± 0.73 a | 2.71 ± 0.62 b | 3.06 ± 0.48 b | 4.91 ± 0.66 a | 3.13 ± 0.44 b |
| Cys | 2.15 ± 0.62 b | 0.90 ± 0.28 c | 1.10 ± 0.26 c | 0.34 ± 0.07 d | 0.20 ± 0.05 d | 2.91 ± 0.19 a |
| Val | 3.66 ± 0.17 bc | 4.27 ± 0.58 b | 3.41 ± 0.29 c | 3.66 ± 0.36 bc | 5.34 ± 0.16 a | 5.12 ± 0.37 a |
| Met | 0.93 ± 0.25 b | 1.16 ± 0.15 ab | 0.79 ± 0.07 b | 0.81 ± 0.25 b | 1.12 ± 0.11 ab | 1.35 ± 0.29 a |
| Ile | 3.51 ± 0.21 a | 3.78 ± 0.05 a | 2.91 ± 0.18 b | 2.69 ± 0.26 b | 3.43 ± 0.17 a | 3.67 ± 0.31 a |
| Leu | 5.73 ± 0.54 b | 5.73 ± 0.24 b | 4.38 ± 0.12 c | 5.09 ± 0.75 bc | 7.57 ± 1.05 a | 5.75 ± 0.56 b |
| Tyr | 1.58 ± 0.15 cd | 1.59 ± 0.25 cd | 1.21 ± 0.23 d | 1.68 ± 0.33 c | 3.10 ± 0.13 b | 3.83 ± 0.25 a |
| Phe | 3.22 ± 0.09 c | 3.14 ± 0.05 c | 3.51 ± 0.05 b | 2.56 ± 0.28 d | 3.88 ± 0.15 a | 3.62 ± 0.12 ab |
| Lys | 2.20 ± 0.24 a | 1.92 ± 0.12 ab | 1.59 ± 0.25 bc | 1.06 ± 0.15 d | 1.65 ± 0.22 bc | 1.49 ± 0.16 c |
| His | 1.82 ± 0.08 a | 1.35 ± 0.05 c | 1.38 ± 0.28 c | 1.42 ± 0.17 bc | 1.83 ± 0.14 a | 1.73 ± 0.23 ab |
| Arg | 7.13 ± 0.46 a | 6.86 ± 0.15 ab | 4.93 ± 0.86 c | 4.04 ± 0.25 d | 6.52 ± 0.23 ab | 6.12 ± 0.38 b |
| Trp | 1.39 ± 0.21 a | 0.84 ± 0.23 bc | 1.10 ± 0.11 ab | 0.48 ± 0.12 c | 1.33 ± 0.25 a | 1.30 ± 0.33 a |
| Pro | 5.89 ± 0.15 a | 3.30 ± 0.07 d | 3.86 ± 0.21 c | 3.75 ± 0.14 b | 4.54 ± 0.09 b | 3.56 ± 0.26 cd |
| TAAs | 84.32 a | 66.31 c | 72.91 b | 74.12 b | 86.83 a | 82.28 a |
| AAAs | 6.19 b | 5.57 c | 4.82 cd | 5.07 c | 8.30 a | 8.75 a |
| BCAAs | 12.91 bc | 13.77 b | 10.70 d | 11.43 c | 16.33 a | 14.54 b |
| HAAs | 24.63 b | 24.94 b | 19.15 c | 19.53 c | 28.22 a | 23.37 b |
| PCAAs | 11.15 a | 10.13 ab | 7.90 c | 6.52 d | 9.99 ab | 9.34 b |
| NCAAs | 32.37 b | 19.00 d | 32.48 b | 33.18 a | 28.41 c | 28.96 c |
| EAAs | 27.22 b | 25.02 c | 20.59 d | 19.96 d | 29.79 a | 27.74 ab |
Asp: Aspartic acid; Thr: Threonine; Ser: Serine; Glu: Glutamic acid; Gly: Glycine; Ala: Alanine; Cys: Cysteine; Val: Valine; Met: Methionine; Ile: Isoleucine; Leu: Leucine; Tyr: Tyrosine; Phe: Phenylalanine; Lys: Lysine; His: Histidine; His: Histidine; Arg: Arginine; Trp: Tryptophan; Pro: Proline. Results are expressed as the mean ± standard deviation (n = 3). Different letters in the same row correspond to a significant difference at p < 0.05. Aromatic amino acids (AAAs) = Phe, Tyr, and Trp; Branch chain amino acids (BCAAs) = Leu, Ile, and Val; Hydrophobic amino acids (HAAs) = Ala, Val, Ile, Leu, Tyr, Phe, Trp, Pro, Met, and Cys; Positively charged amino acids (PCAAs) = Arg, His, and Lys; Negatively charged amino acids (NCAAs) = Asp and Glu.