FIG. 3.

Expression of mtrA in infected macrophages monitored by mtrA-gfp fusion and epifluorescence microscopy. M. tuberculosis H37Rv or M. bovis BCG carrying the pmtrA-gfp or phsp60-gfp reporter plasmid was used to infect macrophages, which were incubated for 2 h, 3 days, or 5 days before the cells were fixed and prepared for fluorescence microscopy analysis. Panels: A to H, J774 murine macrophage-like cell line; I to L, human peripheral blood monocyte-derived macrophages. Arrows point at the bacteria within the macrophages.