Table 2.

Sequences of alleles and primers for vST analysis

List of alleles of vST groups with corresponding accession numbers of reference plasmid sequences and SNPs, and primers for amplifying and sequencing alleles.

Allele	Reference accession no.	Allele SNP	Primer
vapBC1	CP030916	Reference allele	5′-GCGATACTCATCATAAACGTATATCCC-3′ 5′-GTTGAGCCGGGAGATGATTTC-3′
vapBC2	CP037924	A210G
vapBC3	CP024472	G36A; A210G; A322G
vapBC4	CP055125	G36A; A172G; A210G; A322G
vapBC5	CP026804.1	A210G; A322G
vapBC6	CP024476.1	A210G; G242T
parAB1	CP030916.1	Reference allele	5′-GTTAAAATCCTACATAGCACGGAGG-3′ 5′-CGTGAAAGAGGGGGAAGACTATC-3′ 5′-TGCGAATCCTGTTACTTATGTTGG-3′ 5′-CCATCAGTAGTTGGCCCTTATAAATTC-3′
parAB2	CP037924.1	G171C; C519T; T1358C
parAB3	CP026800.1	G171C; T1358C
parAB4	CP055125	T1358C; G1703A
parAB5	CP024472	T1358C
parAB6	CP030773.1	C1811T
parAB7 parAB8	AF386526.1 AAZJOL	T1376C G637T; T1358C
yacAB1	CP030916	Reference allele	5′-GGCATATCCTGGTGACGATATCTG-3′ 5′-ATGGTAGATGTTATTTTCATGTCATACCTTTG-3′
yacAB2	CP037924	T175C
yacAB3	CP026099	T175C; A392G