Table 2.
Qualitative and quantitative phytochemical characterization of flavan-3-ols and proanthocyanidins in WRSP extract. Results (mg per 100 g of FW) are expressed as mean ± SD of three different experiments.
| # | RT [Min] | M-H | MS/MS | CAS-ID | Chemical Formula |
Compound(s) | mg/100 g | ||
|---|---|---|---|---|---|---|---|---|---|
| Flavan-3-ols | |||||||||
| 3 | 14.2 | 451 | 289 | n.a. | C21H24O11 | Catechin-3-O-galactoside | 0.75 ± 0.03 | ||
| 4 | 14.9 | 451 | 289 | n.a. | C21H24O11 | Catechin-3-O-glucoside | 1.32 ± 0.03 | ||
| 5 | 15.6 | 597 | 451 | 435 | 289 | n.a. | C27H34O15 | Catechin-3-O-rutinoside | 7.34 ± 0.18 |
| 21 | 29.7 | 289 | 154-23-4 | C15H14O6 | Catechin | 122 ± 3.48 | |||
| 36 | 51.2 | 613 | 451 | 289 | n.a. | C27H34O16 | Catechin-3,7-O-diglucoside | 2.46 ± 0.14 | |
| Proanthocyanidins | |||||||||
| 8 | 22.8 | 577 | 289 | 29,106-49-8 | C30H26O12 | Dimer B-Type PAC | 399.61 ± 5.95 | ||
| 13 | 24.9 | 865 | 575 | 289 | 65,085-09-8 | C45H38O18 | Trimer A-Type PAC | 14.26 ± 0.17 | |
| 17 | 27.5 | 1153 | 867 | 577 | n.a. | C60H52O24 | Tetramer B-Type PAC | 12.63 ± 0.41 | |
| 23 | 31.8 | 1439 | 1437 | 1151 | 575 | n.a. | C75H60O30 | Pentamer A-Type PAC | 56.16 ± 3.83 |
| 28 | 37.2 | 2012 | 1151 | 863 | 289 | n.a. | C90H72O36 | Esamer A-Type PAC | 12.05 ± 0.5 |
| 29 | 37.2 | 2014 | 1153 | 861 | 577 | n.a. | C90H78O36 | Esamer B-Type PAC | 5.16 ± 0.17 |
| 30 | 37.5 | 1437 | 1151 | 863 | 575 | n.a. | C75H60O30 | Pentamer A-Type PAC | 30.88 ± 1.58 |
| 31 | 38.8 | 1151 | 863 | 575 | 289 | n.a. | C60H48O24 | Tetramer A-Type PAC | 13.35 ± 0.64 |
| 32 | 42.5 | 1441 | 1155 | 865 | 289 | n.a. | C75H65O30 | Pentamer B-Type PAC | 77.33 ± 3.16 |
RT: retention time; m/z: mass-to-charge ratio; MS/MS: detected fragmentations; CAS-ID: Chemical Abstracts Service Identification Number; n.a.: not available.