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. 2022 Mar 31;16(9):1461–1474. doi: 10.1093/ecco-jcc/jjac049

Figure 3.

Figure 3.

IL10 secretion by iNKT is required to suppress colitogenic CD4+T cells. [A] Cumulative histological scores, [B] colon length, [C] Il1β colonic expression, [D] Ki67+CD4+ T cells absolute numbers, and [E] frequency of IFNγ-producing colonic CD4+ T cells in colon specimens of the indicated experimental groups. Statistical significance was calculated using the Kruskal-Wallis test corrected for multiple comparisons by controlling the false-discovery rate [FDR]; *p ≤0.05, **p ≤0.01, ***p ≤0.001. [F] PCoA of microbiota beta-diversity as measured by Weighted UniFrac distance. [G] Volcano plot representing the significantly enriched bacterial taxa [FDR p <0.05] in transplanted IL10+/+ vs IL10-/- iNKT animals by DEseq2 analysis. The names of the significantly enriched amplicon sequence variants [ASVs] classified to the genus and family levels are reported. [H] Relative abundance of the significantly enriched SCFA-producing bacteria identified by DEseq2 analysis in mice injected with naïve CD4+T, IL10-deficient iNKT [+iNKT IL10-/-] and IL10-proficient iNKT [+iNKT IL10+/+] cells. [I] Representative dot plots and cumulative graphs of IL10 production by murine colonic iNKT cells upon in vivo exposure to SCFAs. Statistical significance was calculated using the Kruskal-Wallis test corrected for multiple comparisons by controlling the false-discovery rate [FDR]; *p ≤0.05. SCFA, short chain fatty acid.